The Pueblo Railway Museum is managed by the all-volunteer, not-for-profit Pueblo Railway Foundation, which was formed in 2003 to continue the activities of the Pueblo Locomotive & Rail Historical Society. The PL&RHS started the collection, but has been disbanded.
The collection is located on W B St next to the old Pueblo Union Depot in the Union Avenue National Historic District of downtown Pueblo, CO. It is about a one hundred mile drive south from Denver on the I-25. The museum seeks to preserve historic railroad equipment and promote interest in railroading, railroad history and the impact of the railroad on the history of Pueblo and the United States.
Pueblo has been a major rail hub since the late 1800s, and the arrival of the railroads contributed to the growth of the city into the largest industrial center west of the Mississippi from 1880 to about 1940. The collection specialises in equipment from railroads that served the city prior to their consolidation into the BNSF and Union Pacific, and includes the only surviving Colorado & Wyoming locomotives and cars.